| 01 Sept 22 | F. Grillitsch Florian Grillitsch | XXX | → |  Ajax AmsterdamAJA | Free |
| 01 Sept 22 | Antony | Ajax AmsterdamAJA | → |  Manchester UnitedMAN | 95000000 |
| 31 Aug 22 | L. Ocampos Lucas Ocampos |  SevillaSEV | → | Ajax AmsterdamAJA | Loan |
| 30 Aug 22 | N. Ünüvar Naci Ünüvar | Ajax AmsterdamAJA | → |  TrabzonsporTRAB | Loan |
| 29 Aug 22 | S. Klaiber Sean Klaiber | Ajax AmsterdamAJA | → |  FC UtrechtUTR | Undisclosed |
| 19 Aug 22 | A. Kaplan Ahmetcan Kaplan | TrabzonsporTRAB | → | Ajax AmsterdamAJA | Undisclosed |
| 18 Aug 22 | P. Schuurs Perr Schuurs | Ajax AmsterdamAJA | → |  TorinoTOR | 9000000 |
| 10 Aug 22 | J. Sánchez Jorge Sánchez |  AméricaAME | → | Ajax AmsterdamAJA | Undisclosed |
| 04 Aug 22 | L. Lucca Lorenzo Lucca |  PisaPIS | → | Ajax AmsterdamAJA | Loan |
| 03 Aug 22 | M. Daramy Mohamed Daramy | Ajax AmsterdamAJA | → |  FC CopenhagenCPH | Loan |
| 27 Jul 22 | L. Martínez Lisandro Martínez | Ajax AmsterdamAJA | → | Manchester UnitedMAN | 57370000 |
| 24 Jul 22 | N. Tagliafico Nicolás Tagliafico | Ajax AmsterdamAJA | → |  LyonLYON | 4200000 |
| 22 Jul 22 | B. Brobbey Brian Brobbey |  RB LeipzigRBL | → | Ajax AmsterdamAJA | Undisclosed |
| 21 Jul 22 | F. Conceição Francisco Conceição |  FC PortoPOR | → | Ajax AmsterdamAJA | Undisclosed |
| 20 Jul 22 | C. Bassey Calvin Bassey |  RangersRAN | → | Ajax AmsterdamAJA | 23000000 |
| 12 Jul 22 | O. Wijndal Owen Wijndal |  AZ AlkmaarAZ | → | Ajax AmsterdamAJA | 10,000,000 |
| 08 Jul 22 | S. Bergwijn Steven Bergwijn |  Tottenham HotspurTOT | → | Ajax AmsterdamAJA | Undisclosed |
| 06 Jul 22 | S. Haller Sébastien Haller | Ajax AmsterdamAJA | → |  Borussia DortmundDOR | Undisclosed |
| 01 Jul 22 | L. van Gelderen Liam van Gelderen | Ajax AmsterdamAJA | → |  FC GroningenGRO | Undisclosed |
| 01 Jul 22 | Danilo | Ajax AmsterdamAJA | → |  Feyenoord RotterdamFEY | Free |
| 01 Jul 22 | N. Musampa Nordin Musampa | Ajax AmsterdamAJA | → | FC GroningenGRO | Free |
| 01 Jul 22 | D. Kotarski Dominik Kotarski | Ajax AmsterdamAJA | → | HGXHNK GoricaHNK Gorica | Undisclosed |
| 01 Jul 22 | A. Onana Andre Onana | Ajax AmsterdamAJA | → |  InternazionaleINT | Free |
| 01 Jul 22 | R. Gravenberch Ryan Gravenberch | Ajax AmsterdamAJA | → |  Bayern MunichMUN | 18500000 |
| 01 Jul 22 | N. Mazraoui Noussair Mazraoui | Ajax AmsterdamAJA | → | Bayern MunichMUN | Free |
| 07 Mar 22 | P. Tyton Przemyslaw Tyton | XXX | → | Ajax AmsterdamAJA | Free |
| 30 Jan 22 | M. Ihattaren Mohamed Ihattaren |  JuventusJUV | → | Ajax AmsterdamAJA | Loan |
| 14 Jan 22 | D. Neres David Neres | Ajax AmsterdamAJA | → | SDXShakhtar DonetskShakhtar Donetsk | 12000000 |
| 04 Jan 22 | B. Brobbey Brian Brobbey | RB LeipzigRBL | → | Ajax AmsterdamAJA | Loan |
| 04 Jan 22 | M. de Waal Max de Waal | Ajax AmsterdamAJA | → |  PEC ZwollePEC | Loan |