| 02 Sept 25 | K. Dolberg Kasper Dolberg |  AnderlechtAND | → |  Ajax AmsterdamAJA | Undisclosed |
| 01 Sept 25 | B. Traoré Bertrand Traoré | Ajax AmsterdamAJA | → |  SunderlandSUN | Undisclosed |
| 01 Sept 25 | B. Brobbey Brian Brobbey | Ajax AmsterdamAJA | → | SunderlandSUN | 20000000 |
| 29 Aug 25 | A. Kaplan Ahmetcan Kaplan | Ajax AmsterdamAJA | → |  FC GroningenGRO | Loan |
| 29 Aug 25 | J. McConnell James McConnell |  LiverpoolLIV | → | Ajax AmsterdamAJA | Loan |
| 29 Aug 25 | J. Faberski Jan Faberski | Ajax AmsterdamAJA | → |  PEC ZwollePEC | Loan |
| 29 Aug 25 | T. Gooijer Tristan Gooijer | Ajax AmsterdamAJA | → | PEC ZwollePEC | Loan |
| 20 Aug 25 | S. Mannsverk Sivert Mannsverk | Ajax AmsterdamAJA | → | SPXSparta PragueSparta Prague | Loan |
| 19 Aug 25 | D. Janse Dies Janse | Ajax AmsterdamAJA | → | FC GroningenGRO | Loan |
| 10 Aug 25 | C. Akpom Chuba Akpom | Ajax AmsterdamAJA | → |  Ipswich TownIPS | Loan |
| 08 Aug 25 | K. Itakura Kou Itakura |  Borussia MönchengladbachBMG | → | Ajax AmsterdamAJA | Undisclosed |
| 03 Aug 25 | J. Hato Jorrel Hato | Ajax AmsterdamAJA | → |  ChelseaCHE | 44180000 |
| 30 Jul 25 | O. Gloukh Oscar Gloukh |  RB SalzburgSLZ | → | Ajax AmsterdamAJA | 14750000 |
| 23 Jul 25 | J. Gorter Jay Gorter | Ajax AmsterdamAJA | → | PFXPafos FCPafos FC | Free |
| 17 Jul 25 | C. Forbs Carlos Forbs | Ajax AmsterdamAJA | → |  Club BruggeBRU | Undisclosed |
| 15 Jul 25 | J. Henderson Jordan Henderson | Ajax AmsterdamAJA | → |  BrentfordBRE | Free |
| 15 Jul 25 | R. Moro Raúl Moro |  Real ValladolidVLL | → | Ajax AmsterdamAJA | Undisclosed |
| 10 Jul 25 | C. Rasmussen Christian Rasmussen | Ajax AmsterdamAJA | → |  Fortuna DüsseldorfF95 | Undisclosed |
| 01 Jul 25 | J. Medic Jakov Medic | Ajax AmsterdamAJA | → |  Norwich CityNOR | Undisclosed |
| 01 Jul 25 | J. Rijkhoff Julian Rijkhoff | Ajax AmsterdamAJA | → |  Almere CityALM | Loan |
| 01 Jul 25 | V. Jaros Vitezslav Jaros | LiverpoolLIV | → | Ajax AmsterdamAJA | Loan |
| 04 Feb 25 | Matheus |  BragaSCB | → | Ajax AmsterdamAJA | Loan |
| 04 Feb 25 | L. Rosa Lucas Rosa | Real ValladolidVLL | → | Ajax AmsterdamAJA | Undisclosed |
| 03 Feb 25 | D. Ramaj Diant Ramaj | Ajax AmsterdamAJA | → |  Borussia DortmundDOR | Undisclosed |
| 03 Feb 25 | B. Tahirovic Benjamin Tahirovic | Ajax AmsterdamAJA | → |  Brøndby IFBRO | Undisclosed |
| 03 Feb 25 | J. Banel Jaydon Banel | Ajax AmsterdamAJA | → |  BurnleyBUR | Undisclosed |
| 02 Feb 25 | C. Akpom Chuba Akpom | Ajax AmsterdamAJA | → |  LilleLILL | Loan |
| 31 Jan 25 | K. Hlynsson Kristian Hlynsson | Ajax AmsterdamAJA | → |  Sparta RotterdamSPA | Loan |
| 31 Jan 25 | O. Edvardsen Oliver Edvardsen |  Go Ahead EaglesGAE | → | Ajax AmsterdamAJA | Undisclosed |
| 28 Jan 25 | S. Mannsverk Sivert Mannsverk | Ajax AmsterdamAJA | → |  Cardiff CityCAR | Loan |
| 28 Jan 25 | S. Mannsverk Sivert Mannsverk | Ajax AmsterdamAJA | → | Cardiff CityCAR | Loan |
| 24 Jan 25 | Y. Regeer Youri Regeer |  FC TwenteTWE | → | Ajax AmsterdamAJA | Undisclosed |
| 23 Jan 25 | D. Rensch Devyne Rensch | Ajax AmsterdamAJA | → |  AS RomaROMA | Undisclosed |
| 18 Jan 25 | G. Ávila Gastón Ávila | Ajax AmsterdamAJA | → |  FortalezaFOR | Loan |
| 16 Jan 25 | N. Ünüvar Naci Ünüvar | Ajax AmsterdamAJA | → | FC TwenteTWE | Free |